Dr. Balentine received his undergraduate degree from McDaniel College in Westminster, Maryland. He attended medical school at the Philadelphia College of Osteopathic Medicine graduating in 1983. He completed his internship at St. Joseph's Hospital in Philadelphia and his Emergency Medicine residency at Lincoln Medical and Mental Health Center in the Bronx, where he served as chief resident. In 1992 Dr. Balentine started practicing Emergency Medicine at St. Barnabas Hospital in the Bronx where he served as Residency Director and Department Co-Director.

Dr. Balentine served as Chief Medical Officer and Executive Vice President of St. Barnabas Hospital and Healthcare System in the Bronx until 2014. He is Past-President of the New York Chapter of the American College of Emergency Physicians and Vice Chair of the New York State Board of Medicine. He has authored and edited many web and textbook chapters and was the founding medical editor of newyorkmedicaljournal.org.

Dr. Balentine is currently Dean, College of Osteopathic Medicine; Vice President, Health Sciences and Medical Affairs at the New York Institute of Technology.